Nicolau Institute of Virology, Bucharest, Romania.
Introduction: In human cancer cells DNMTs are responsible for both de novo and maintenance methylation of tumor suppressor genes. Design: 49 patients aged 1082 years hospitalized for thyroidectomy were included between January and July The inclusion criterion was patients with thyroid nodules with indication for surgery and the exclusion criteria were: hyperthyroidism, medullary thyroid carcinoma, thyroid metastasis, other thyroid tumors, and anaplastic thyroid carcinoma.
Patients were divided into three groups. Group 1: 26 subjects with papillary thyroid carcinoma.
Group 2: 14 patients with follicular adenoma. Group 3: nine patients with multinodular goiter. Group 1 was divided in: Group 1.
Total RNA was isolated from tissues and reverse transcribed. Double normalization was performed related to gene expression levels found in peritumoral tissues.
SPSS 18 program was used to perform statistical analyze. The comparison of cancer subtypes did not reveal significant differences for DNMT1. DNMT1 expression did not correlate with tumor stage, tumor multifocality, capsular, vascular or lymphatic invasion or with metastasis.
Conclusion: DNMT1 is not overexpressed in papillary thyroid carcinoma and does not correlate with tumor stage, tumor multifocality, capsular, vascular or lymphatic invasion or with metastasis. This Volume.